This track displays human tissue microarray data using
Affymetrix Human Exon 1.0 GeneChip.
The track was produced as part of the ENCODE Project.
Release Notes
This is release 2 of this track. This release includes 28 new cell types, and replaces the data for four existing tables (replicate 1 for K562, NB4, and SKMC; replicate 2 for HeLa-S3).
Display Conventions and Configuration
The display for this track shows probe location and signal value
as grayscale-colored items where higher
signal values correspond to darker-colored blocks. Items with score of 1000
are in the highest 10% quantile for signal value of that particular cell type.
Similarly, items scoring 900 are the next 10% quantile and at the bottom of
scale, items scoring 100 are in the lowest 10% quantile for signal value.
The subtracks within this composite annotation track correspond to data from
different cell types and tissues. The configuration options are
shown at the top of the track description page, followed by a list of
subtracks.
To display only selected subtracks, uncheck the boxes next to the tracks you
wish to hide.
For information regarding specific microarray probes, turn on the
Affy Exon Probes track, which can be found inside the
Affy Exon supertrack in the "Expression" track group.
Methods
Cells were grown according to the approved
ENCODE cell culture protocols.
A subset of the cells was stored frozen in RNALater. Total RNA was labeled and
hybridized to Affymetrix Human Exon 1.0 ST arrays. Exon and gene level
expression analysis were carried out using Affymetrix ExACT 1.2.1 and Affymetrix
Expression Console 1.1 software tools. Samples were quantile normalized for
background correction and Probe Logarithmic Intensity Error summarized.
More detailed methods are
here.
Verification
Data were verified by sequencing biological replicates displaying correlation
coefficient >0.9.
Credits
These data were generated by the University of Washington ENCODE group.
Data users may freely use ENCODE data, but may not, without prior
consent, submit publications that use an unpublished ENCODE dataset until
nine months following the release of the dataset. This date is listed in
the Restricted Until column, above. The full data release policy
for ENCODE is available
here.