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Data last updated: 2011-12-31; REBASE version 201
Description
This track shows restriction enzyme recognition sites. At different browser
zoom levels (categorized in the table below by the number of bases visible),
different sets of enzymes are shown. "Base-level" refers to the
browser zoom level at which individual bases are displayed in the Base
Position track. Displays of custom enzyme sets override the table: in these
cases, the restriction sites are consistent across all zoom
levels. "Primary" enzymes are the canonical isoschizomer for each
cutsite, usually the first isolated or most readily available.
The base-level display shows the detail of the cutting pattern of the enzyme as
well as the presence of any ambiguous or redundant nucleotides in the cutsite.
The four standard bases, G, A, T and C, are displayed in black, whereas non-unique
bases using
IUPAC
designations (R, Y, N, etc) are displayed in blue.
The term "isoligamer" in the enzyme details section
was coined to designate enzymes that produce
single-stranded overhanging "sticky" ends that are ligatable to
each other.
Display
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Zoom level (in bases)
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Enzyme set displayed
|
| Base-level |
All "primary" enzymes from REBASE |
| Base-level - 2,999 |
Restriction sites for
"primary" enzymes five bases and larger. |
| 3,000 - 19,999 |
Restriction sites for
"primary" enzymes six bases and larger |
| 20,000 - 249,999 |
The following set of
commonly-used enzymes: ClaI, BamHI, BglII, EcoRI, EcoRV, HindIII, PstI, SalI,
SmaI, XbaI, KpnI, SacI, SphI |
| > 250,000 |
None displayed |
Credits
Thanks to
REBASE
for providing these data.
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