Overview

This super-track combines related tracks of DNaseI sensitivity data from University of Washington (UW).

DNaseI has long been used to map general chromatin accessibility and the DNaseI "hyperaccessibility" or "hypersensitivity" that is a universal feature of active cis-regulatory sequences. The use of this method has led to the discovery of functional regulatory elements that include enhancers, insulators, promotors, locus control regions and novel elements. DNaseI hypersensitivity signifies chromatin accessibility following binding of trans-acting factors in place of a canonical nucleosome, and is a universal feature of active cis-regulatory sequences in vivo.

These tracks contain DNaseI analysis of multiple cell lines using the QCP method or DNaseI-chip.

Credits

Data generation, analysis, and validation were performed by the following members of the ENCODE group at UW in Seattle.

UW Medical Genetics: Patrick Navas, Man Yu, Hua Cao, Brent Johnson, Ericka Johnson, Tristan Frum, and George Stamatoyannopoulos.

UW Genome Sciences: Michael O. Dorschner, Richard Humbert, Peter J. Sabo, Scott Kuehn, Robert Thurman, Anthony Shafer, Jeff Goldy, Molly Weaver, Andrew Haydock, Kristin Lee, Fidencio Neri, Richard Sandstrom, Shane Neff, Brendan Henry, Michael Hawrylycz, Janelle Kawamoto, Paul Tittel, Jim Wallace, William S. Noble, and John A. Stamatoyannopoulos.

References

Dorschner MO, Hawrylycz M, Humbert R, Wallace JC, Shafer A, Kawamoto J, Mack J, Hall R, Goldy J, Sabo PJ et al. High-throughput localization of functional elements by quantitative chromatin profiling. Nat Methods. 2004 Dec;1(3):219-25.

Sabo PJ, Kuehn MS, Thurman R, Johnson BE, Johnson EM, Cao H, Yu M, Rosenzweig E, Goldy J, Haydock A et al. Genome-scale mapping of DNase I sensitivity in vivo using tiling DNA microarrays. Nat Methods. 2006 Jul;3(7):511-8.