Description

This track shows gene trap sequence tags from GenBank dbGSS that have an RNA-based sequence tag method (5'-RACE or 3'-RACE) or DNA-based sequence tag method. DNA-based gene trap sequence tags (purple) are derived from genomic DNA at the site of gene trap vector insertion. RNA-based gene trap sequence tags are derived from the exon adjacent to the insertion site. If the sequence tag method is 5'-RACE (blue), the insertion site is in the intron downstream from the most 3' exon represented in the sequence tag. If the sequence tag is 3'-RACE (green), the insertion site is in the intron upstream from the most 5' exon represented in the sequence tag.

Gene trap mutagenesis requires that the vector inserts in the correct transcriptional orientation of the "trapped" gene. Sequence tags that overlap known genes in the opposite transcriptional orientation do not disrupt those genes from a gene trapping mechanism.

Methods

Sequence tags were collected from dbGSS and aligned to the reference genome by Mouse Genome Informatics (MGI) at The Jackson Laboratory.

Credits

Thanks to MGI at The Jackson Laboratory, and Bob Sinclair in particular, for providing these data.